FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874192
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC1501.3631072E-510.9986098
TAGACTG3000.010.9984185
TAGACAG5050.010.9885195
CGCGCAC1408.1920516E-510.71311810
AGACCGC2251.1044904E-810.66513256
GACTGCT3401.8189894E-1210.1455997
GACCGCG1809.274056E-69.9985617
CCTACAC2609.922587E-99.8062813
CGGCTGG1851.2790804E-59.73154630
CTAATAC7300.09.6561453
CCGCGCA1409.7299734E-49.6416399
TACTATG6950.09.4950377
TCTAGAC3801.8189894E-129.4723223
ACTATGC5900.09.405598
ATCACAC4500.09.33523929
CTTAGGG2251.3506396E-69.3319913
CTATGCA6350.09.2114339
TATACTG7200.09.1653485
TAGGTAG3801.0913936E-119.0776425
GTCCTAG2157.629709E-69.070831
Produced by FastQC (version 0.11.2)