FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309559
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23100.1763952597782918No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCG13230.10102637605483983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTCG350.003007751721.42688210
TAGGACT1251.13421265E-714.3988654
CAGGACC851.6348141E-414.1165334
GTGCGAC2800.013.93066429
TTAGGAC2051.4551915E-1113.1701963
TCAGGAC1151.0936819E-513.0429473
TAGGTAG2001.2732926E-1012.7489945
GTATTAG3650.012.7426141
AGACTGT1804.6766218E-912.4990146
GGTAGTC2051.9281288E-1012.4380437
TGCGACG3150.012.382339530
GTGTGCT850.00261414312.3519679
TAGAGCG1354.212743E-612.2212587
CTACCCC1252.5763593E-511.9990544
GGTGCGA3400.011.47187328
CCTTGCG1703.7664176E-711.4696849
GCCCTAT1059.693954E-411.4311621
AGAACGG4400.011.24954121
TAATACT6150.011.2186284
GTATATA14100.011.1727451
Produced by FastQC (version 0.11.2)