FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length36
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAGATATTGGTAAATCATCTATCGTTGTTTCA27.6923076923076925No Hit
ATGCAAATGGACCAGAGCGATCCAAACGACCCAAAG13.8461538461538463No Hit
TTCAATATGTCCCTCAAAAGATCTCTGATTAAAGAC13.8461538461538463No Hit
TTATAGATCGATTCGCCGTGACGGGAGAGCCAGATA13.8461538461538463No Hit
ATTCTTGACGTTCCGCTGATATTTTGTGTTGGGGTT13.8461538461538463No Hit
CATCAACGCTATCACTTTCTGTTCACAAAGTATGCG13.8461538461538463No Hit
GGTACGTATTATGCAGGGACTTATGGTACGGATGTT13.8461538461538463No Hit
GTCCCAGTAGACATCATGATAGTAAAGAATCTTTTG13.8461538461538463No Hit
TTTGAAGATAGCATCGGGCTGGCTACCATATTAAAT13.8461538461538463No Hit
ATTGTACGATCTGACTAAGTTATCTTTTAATAATTG13.8461538461538463No Hit
TCCTTGAGGTTAATTGCTCAGGCCCTTCGTAGGTCT13.8461538461538463No Hit
GTAGTGGAAGATGAGATAACCTTATGCCTGCTAATT13.8461538461538463No Hit
TGCAGAAACAGGTAAACGCTTGGAAAACCCAAAGAT13.8461538461538463No Hit
CTATTGAAGGATTACAGAAAGCATGGTTCGACCGAG13.8461538461538463No Hit
GGGAATCGATGATAGAGACTTTGACTGAATACGACG13.8461538461538463No Hit
CATGGAAACTTATCGTGGCGTTAATATTGGCACCAA13.8461538461538463No Hit
TTATATATTATCTAACATCAGATGGTTTGTCTTTCA13.8461538461538463No Hit
ACCTAATACTAAGCAAAAATGTAAAATCAAAACACA13.8461538461538463No Hit
CTTCATTACCACCTGTTACCAGTGCGACAGCAAGTC13.8461538461538463No Hit
GGGGTATATTCTGCTGTTGATTTGGATAATGGTGAG13.8461538461538463No Hit
CTTTCATGGTTTGTATTCACACTGAAAATCAAAATC13.8461538461538463No Hit
TAGTAAACTGAGGAGCGCATAACCTAAGTCCCATAG13.8461538461538463No Hit
AAGTCAGAAAGTCTATGGAAAACTACTTTCTGGCTT13.8461538461538463No Hit
AGACAAGAGAGCAAGAAAAAGGAAAAACTTCCTTTC13.8461538461538463No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)