##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	HK5NHBGXX_combined_n02_ntr_3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26
Sequences flagged as poor quality	0
Sequence length	36
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.192307692307693	0.0	0.0	0.0	0.0	0.0
2	32.0	0.0	0.0	0.0	0.0	0.0
3	32.0	0.0	0.0	0.0	0.0	0.0
4	31.307692307692307	0.0	0.0	0.0	0.0	0.0
5	30.192307692307693	0.0	0.0	0.0	0.0	0.0
6	34.30769230769231	0.0	0.0	0.0	0.0	0.0
7	34.84615384615385	0.0	0.0	0.0	0.0	0.0
8	33.96153846153846	0.0	0.0	0.0	0.0	0.0
9	32.96153846153846	0.0	0.0	0.0	0.0	0.0
10	35.84615384615385	0.0	0.0	0.0	0.0	0.0
11	33.30769230769231	0.0	0.0	0.0	0.0	0.0
12	33.46153846153846	0.0	0.0	0.0	0.0	0.0
13	33.73076923076923	0.0	0.0	0.0	0.0	0.0
14	35.15384615384615	0.0	0.0	0.0	0.0	0.0
15	35.23076923076923	0.0	0.0	0.0	0.0	0.0
16	34.0	0.0	0.0	0.0	0.0	0.0
17	34.80769230769231	0.0	0.0	0.0	0.0	0.0
18	34.84615384615385	0.0	0.0	0.0	0.0	0.0
19	35.69230769230769	0.0	0.0	0.0	0.0	0.0
20	33.11538461538461	0.0	0.0	0.0	0.0	0.0
21	32.69230769230769	0.0	0.0	0.0	0.0	0.0
22	35.34615384615385	0.0	0.0	0.0	0.0	0.0
23	35.0	0.0	0.0	0.0	0.0	0.0
24	34.15384615384615	0.0	0.0	0.0	0.0	0.0
25	34.15384615384615	0.0	0.0	0.0	0.0	0.0
26	34.53846153846154	0.0	0.0	0.0	0.0	0.0
27	33.5	0.0	0.0	0.0	0.0	0.0
28	35.0	0.0	0.0	0.0	0.0	0.0
29	30.76923076923077	0.0	0.0	0.0	0.0	0.0
30	33.15384615384615	0.0	0.0	0.0	0.0	0.0
31	32.46153846153846	0.0	0.0	0.0	0.0	0.0
32	30.615384615384617	0.0	0.0	0.0	0.0	0.0
33	32.11538461538461	0.0	0.0	0.0	0.0	0.0
34	35.15384615384615	0.0	0.0	0.0	0.0	0.0
35	34.15384615384615	0.0	0.0	0.0	0.0	0.0
36	33.92307692307692	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
12602	1	0.0
12602	2	0.0
12602	3	0.0
12602	4	0.0
12602	5	0.0
12602	6	0.0
12602	7	0.0
12602	8	0.0
12602	9	-11.0
12602	10	-2.0
12602	11	0.0
12602	12	0.0
12602	13	0.0
12602	14	0.0
12602	15	0.0
12602	16	0.0
12602	17	2.0
12602	18	2.0
12602	19	-2.0
12602	20	0.0
12602	21	-11.0
12602	22	4.5
12602	23	0.0
12602	24	0.0
12602	25	0.0
12602	26	-2.0
12602	27	0.0
12602	28	0.0
12602	29	11.0
12602	30	2.0
12602	31	-11.0
12602	32	9.0
12602	33	2.0
12602	34	0.0
12602	35	-11.0
12602	36	2.0
13202	1	0.0
13202	2	0.0
13202	3	0.0
13202	4	0.0
13202	5	0.0
13202	6	0.0
13202	7	0.0
13202	8	0.0
13202	9	11.0
13202	10	2.0
13202	11	0.0
13202	12	0.0
13202	13	0.0
13202	14	0.0
13202	15	0.0
13202	16	0.0
13202	17	-2.0
13202	18	-2.0
13202	19	2.0
13202	20	0.0
13202	21	11.0
13202	22	-4.5
13202	23	0.0
13202	24	0.0
13202	25	0.0
13202	26	2.0
13202	27	0.0
13202	28	0.0
13202	29	-11.0
13202	30	-2.0
13202	31	11.0
13202	32	-9.0
13202	33	-2.0
13202	34	0.0
13202	35	11.0
13202	36	-2.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	1.0
27	0.0
28	2.0
29	0.0
30	2.0
31	0.0
32	2.0
33	3.0
34	6.0
35	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.923076923076923	26.923076923076923	26.923076923076923	19.230769230769234
2	23.076923076923077	15.384615384615385	53.84615384615385	7.6923076923076925
3	26.923076923076923	23.076923076923077	30.76923076923077	19.230769230769234
4	26.923076923076923	15.384615384615385	34.61538461538461	23.076923076923077
5	7.6923076923076925	53.84615384615385	23.076923076923077	15.384615384615385
6	19.230769230769234	61.53846153846154	19.230769230769234	0.0
7	34.61538461538461	34.61538461538461	19.230769230769234	11.538461538461538
8	23.076923076923077	53.84615384615385	19.230769230769234	3.8461538461538463
9	26.923076923076923	19.230769230769234	26.923076923076923	26.923076923076923
10	26.923076923076923	30.76923076923077	38.46153846153847	3.8461538461538463
11	19.230769230769234	42.30769230769231	26.923076923076923	11.538461538461538
12	11.538461538461538	15.384615384615385	46.15384615384615	26.923076923076923
13	34.61538461538461	15.384615384615385	26.923076923076923	23.076923076923077
14	7.6923076923076925	38.46153846153847	26.923076923076923	26.923076923076923
15	38.46153846153847	23.076923076923077	15.384615384615385	23.076923076923077
16	19.230769230769234	42.30769230769231	23.076923076923077	15.384615384615385
17	30.76923076923077	26.923076923076923	26.923076923076923	15.384615384615385
18	30.76923076923077	42.30769230769231	15.384615384615385	11.538461538461538
19	11.538461538461538	46.15384615384615	11.538461538461538	30.76923076923077
20	11.538461538461538	30.76923076923077	34.61538461538461	23.076923076923077
21	26.923076923076923	42.30769230769231	26.923076923076923	3.8461538461538463
22	23.076923076923077	15.384615384615385	46.15384615384615	15.384615384615385
23	11.538461538461538	23.076923076923077	34.61538461538461	30.76923076923077
24	23.076923076923077	30.76923076923077	30.76923076923077	15.384615384615385
25	19.230769230769234	46.15384615384615	19.230769230769234	15.384615384615385
26	19.230769230769234	38.46153846153847	30.76923076923077	11.538461538461538
27	15.384615384615385	23.076923076923077	38.46153846153847	23.076923076923077
28	19.230769230769234	34.61538461538461	19.230769230769234	26.923076923076923
29	19.230769230769234	30.76923076923077	38.46153846153847	11.538461538461538
30	23.076923076923077	11.538461538461538	42.30769230769231	23.076923076923077
31	19.230769230769234	30.76923076923077	15.384615384615385	34.61538461538461
32	15.384615384615385	42.30769230769231	23.076923076923077	19.230769230769234
33	19.230769230769234	34.61538461538461	30.76923076923077	15.384615384615385
34	30.76923076923077	26.923076923076923	34.61538461538461	7.6923076923076925
35	0.0	30.76923076923077	42.30769230769231	26.923076923076923
36	30.76923076923077	23.076923076923077	26.923076923076923	19.230769230769234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	1.5
25	3.0
26	1.5
27	0.0
28	0.0
29	0.5
30	1.0
31	1.0
32	2.0
33	3.0
34	3.0
35	3.0
36	3.0
37	3.0
38	2.5
39	2.0
40	4.0
41	6.0
42	6.0
43	5.0
44	4.0
45	4.0
46	2.0
47	0.0
48	0.0
49	2.0
50	4.0
51	2.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	26.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.15384615384616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.0	92.3076923076923
2	4.0	7.6923076923076925
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTGGAAGATATTGGTAAATCATCTATCGTTGTTTCA	2	7.6923076923076925	No Hit
ATGCAAATGGACCAGAGCGATCCAAACGACCCAAAG	1	3.8461538461538463	No Hit
TTCAATATGTCCCTCAAAAGATCTCTGATTAAAGAC	1	3.8461538461538463	No Hit
TTATAGATCGATTCGCCGTGACGGGAGAGCCAGATA	1	3.8461538461538463	No Hit
ATTCTTGACGTTCCGCTGATATTTTGTGTTGGGGTT	1	3.8461538461538463	No Hit
CATCAACGCTATCACTTTCTGTTCACAAAGTATGCG	1	3.8461538461538463	No Hit
GGTACGTATTATGCAGGGACTTATGGTACGGATGTT	1	3.8461538461538463	No Hit
GTCCCAGTAGACATCATGATAGTAAAGAATCTTTTG	1	3.8461538461538463	No Hit
TTTGAAGATAGCATCGGGCTGGCTACCATATTAAAT	1	3.8461538461538463	No Hit
ATTGTACGATCTGACTAAGTTATCTTTTAATAATTG	1	3.8461538461538463	No Hit
TCCTTGAGGTTAATTGCTCAGGCCCTTCGTAGGTCT	1	3.8461538461538463	No Hit
GTAGTGGAAGATGAGATAACCTTATGCCTGCTAATT	1	3.8461538461538463	No Hit
TGCAGAAACAGGTAAACGCTTGGAAAACCCAAAGAT	1	3.8461538461538463	No Hit
CTATTGAAGGATTACAGAAAGCATGGTTCGACCGAG	1	3.8461538461538463	No Hit
GGGAATCGATGATAGAGACTTTGACTGAATACGACG	1	3.8461538461538463	No Hit
CATGGAAACTTATCGTGGCGTTAATATTGGCACCAA	1	3.8461538461538463	No Hit
TTATATATTATCTAACATCAGATGGTTTGTCTTTCA	1	3.8461538461538463	No Hit
ACCTAATACTAAGCAAAAATGTAAAATCAAAACACA	1	3.8461538461538463	No Hit
CTTCATTACCACCTGTTACCAGTGCGACAGCAAGTC	1	3.8461538461538463	No Hit
GGGGTATATTCTGCTGTTGATTTGGATAATGGTGAG	1	3.8461538461538463	No Hit
CTTTCATGGTTTGTATTCACACTGAAAATCAAAATC	1	3.8461538461538463	No Hit
TAGTAAACTGAGGAGCGCATAACCTAAGTCCCATAG	1	3.8461538461538463	No Hit
AAGTCAGAAAGTCTATGGAAAACTACTTTCTGGCTT	1	3.8461538461538463	No Hit
AGACAAGAGAGCAAGAAAAAGGAAAAACTTCCTTTC	1	3.8461538461538463	No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC	1	3.8461538461538463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE