FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404880
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35920.1493629619773128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACG800.001657886513.12440812
GCCGGAC800.001657886513.12440811
GTGCGAC3700.012.97481629
TGCGACG3700.012.97454630
CTAGACT2255.456968E-1212.6653074
GTATTAG7200.012.0865591
CACGCAG4200.011.78591816
CAGCCGG1406.3295174E-611.7844489
GGGGTGC900.004012478511.6654148
TAGACAG3900.011.5372225
TAGGTAG4000.011.2487925
TAGACTG2155.4242264E-911.1615925
CGCCGCG1355.6497825E-511.11176624
CCCGCCT950.00600091711.05236313
GGCGCCG950.006001845511.05213411
GGTAGTC4050.010.7395877
TAGTCAG3950.010.6317699
GCGCTCG1000.00878875710.4988739
ATACGGT6200.010.1601986
TACGGTG5650.010.0874127
Produced by FastQC (version 0.11.2)