FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648954
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38730.14620865443492034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG3650.012.32941151
ACCGTGT1101.0452217E-412.2710518
GTCCTAT2102.8921932E-1012.1434911
TAGACCG1100.001435613410.9073955
CCGAGCC1253.4288497E-410.7985259
GCGCACT1000.00878859110.49896211
AGTGCGC1000.00879105110.49856710
GTCTATA4600.010.4353281
AGGACAG2505.2150426E-910.1984155
GTATAAA6950.010.1444161
CTAGGAC1653.9039565E-59.99844553
GGACAGT2752.3774192E-99.8168416
TAGGGAG2752.3792381E-99.8166555
TAGACAG4300.09.7659245
TTATACT7800.09.6138914
CAGTGCG1300.00573074459.2295089
ACCGTGC1300.00573074459.2295088
TATATAC7650.09.2142543
TACCGTG2505.809743E-78.9987717
TGTACCG2003.226394E-58.9986025
Produced by FastQC (version 0.11.2)