FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3802653
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66690.1753775587727831No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCC900.004012435711.6657168
GACAGCG2651.10958354E-1010.75383957
TATACTG11950.010.29204755
GTATTAG8650.09.8869681
TAGGCTG4750.09.4729125
TAGACAG6750.09.3325735
ATTATAC14050.09.2874993
GTATATA24250.09.1570231
TAGGACA4100.09.1455964
CAGAGCG2301.8197843E-69.129699
CCTATAC5100.09.1169043
GTATTAT15350.08.992571
GTGTAGA10250.08.9291541
TATTATA19500.08.9225842
GTATTAC8750.08.9166081
ACCCCGT1350.00781059038.8881646
GTCCTAT3254.440153E-98.7715141
TCCTATA6300.08.5709552
ATACCGC2803.2209027E-78.570736
TCTTATA10700.08.5509292
Produced by FastQC (version 0.11.2)