FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954740
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42380.21680632718417797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC1059.721529E-411.4278118
GTCCTAT1459.3252675E-611.3808821
GTCTAAG3101.8189894E-1110.1626941
CTTACAC3850.010.1286783
GTCTTAT3451.8189894E-1210.0013821
GTATATA10700.09.814441
TACACAG4200.09.6417225
GTATAAA6300.09.5251251
ACCTAGA2251.3445333E-69.3346221
CTAGACT2752.4745532E-89.2716364
TCTTATA5350.09.2514852
TAGACAG3801.0913936E-119.077885
TAGGGGT1500.00182585028.9989414
CTATACT4850.08.9680174
TACACTG6050.08.924575
TCTAGAC2701.8463288E-78.8878433
TAGGACA1705.7776197E-48.8224914
TCTACAC4450.08.7630143
GCCTTAG2403.2252065E-68.7512081
GTATTAT5900.08.6452621
Produced by FastQC (version 0.11.2)