FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7390847
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99280.1343283117618319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA34000.09.8841991
ACCGCGT1300.00573093639.229878
GGACCGT3654.7293724E-119.04021556
GTATTAT23400.08.9760351
CCGTGCA3552.6011548E-108.8723759
GTATTAG16050.08.69321251
TATTATA30400.08.6342022
TAATACT23250.08.3862794
GTATAAG21700.08.3656181
TAGGTCG2557.3307892E-68.2344365
GTATTAA20100.08.2104881
GTATAAA25050.08.2051261
CTAATAC13800.08.151383
GTATAAC13500.08.1126261
GTATTAC14500.08.0704731
TTATACT27750.08.0532694
TATACAG19350.07.9836645
ATTATAC22300.07.9364463
TAGACAG10850.07.87936355
GTATAAT21800.07.84550241
Produced by FastQC (version 0.11.2)