FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3134134
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56390.17992210926527072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAC3300.010.4539453
GTATATA20150.010.4227341
GTATTAG7150.010.2806061
TATTATA16400.09.87748052
GTATTAT12500.09.8408481
GTACTAA5000.09.6008271
ATTATAC11250.09.1994713
GTATTAC7600.09.0797291
CCCCTAT2003.21782E-59.0007751
ACCGAGG1500.001826558.9989088
TTATACA12350.08.8654634
TTATACT13650.08.6804054
CTTATAC10050.08.6562193
TTAGAAC5400.08.6106173
CCTTATA7400.08.5130232
ACAGTGC5150.08.4455788
CCTAGAC2505.610802E-68.3995173
ATCGGTC2157.531722E-58.3710788
ATACCGT5200.08.3642376
TATACTG10850.08.1556935
Produced by FastQC (version 0.11.2)