FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152303
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCCG400.00651328118.7486157
TAGACAG2009.094947E-1213.4990045
CCTCGCT850.002612863712.3525669
GGCCTGG900.004005404711.6673261
ACCGTGC900.004009302711.6658078
GTCTAGG1059.7062363E-411.4292151
CCGCAGA1059.7132946E-411.42822511
CTTACAC2503.8198777E-1111.3986643
TCTAGGA1202.3466682E-411.2486812
TCTTATA3500.011.1415522
GTCTAGT950.00599410411.0532551
GACCTCG950.005999914411.0518167
TTACACT2957.2759576E-1210.6767154
CTTATAC3250.010.6141413
GTATAGG1851.1189659E-610.5411361
GTCTTAC2453.7161954E-910.408751
TTAGACT2505.1968527E-910.1988064
GTATTAA2802.9831426E-1010.1791461
GTATTTA3001.0004442E-1010.0005651
GTTTTAG1952.1980777E-610.0005651
Produced by FastQC (version 0.11.2)