FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2779765
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40850.14695486848708433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG7500.013.201971
CGCGAGG800.001658043113.12436313
CTAATAC7150.012.3759083
GTATAGA7900.012.3436161
GTATAAT9550.011.9389551
CCTAATA8100.010.9246092
TCTATAC7450.010.8709773
GTATTAT12250.010.777121
TAGGTAG4250.010.5865775
ACCGTGT2701.546141E-1010.5537148
TATACCG5200.010.382995
ACTATGC7800.010.3828038
TATTAGG7150.010.27848152
TACTATG8250.09.9984347
GGTAGTC4100.09.8765027
GGACTGT2609.922587E-99.8061576
TAATACT12300.09.7545714
CTAGGGT1705.4179654E-59.7043644
GCGCCGA2209.903033E-79.54705226
TATACAG11950.09.5382565
Produced by FastQC (version 0.11.2)