FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6440345
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109080.1693698086049738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17850.012.606891
CTAATAC19800.011.9681983
TAGGTAG9350.011.7098535
CCTAGCG4800.011.564733530
CTATGCA15650.011.40459
TAATACT30650.011.3526634
ACTATGC19850.011.2581238
CTAGGAC4150.010.8420153
TATTAGG17050.010.2920472
GTATAGA20200.010.2490261
TCTATAC18050.09.971053
TTAGGTA13150.09.9227894
CCTAATA20500.09.8768882
GTATTAA18800.09.8152681
AATACTA28050.09.7849465
TACACCG7600.09.6699175
ACCTAAT19300.09.6387181
GTATAAA23200.09.5703681
TATACTG27300.09.5593165
TCACACA13300.09.47551330
Produced by FastQC (version 0.11.2)