FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7063978
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127630.18067723313974082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG20350.011.574491
CCGTGCG1602.783202E-510.3112239
GGTAGTC9750.010.3063437
TAGGTAG10550.010.2356265
GTATAGA21900.010.207251
TATTAGG20600.010.120322
TATACTG29100.09.947155
TAATACT35000.09.7701494
CTAATAC23450.09.721613
GTATTAA23500.09.639971
GTATAAT32550.09.4025531
TAGGACC6900.09.34660154
GTATATA59900.09.2420031
TATACAG35100.09.0586435
GTATTAT38100.08.9779351
CTAGGAC5350.08.9708513
TAGTCAG10900.08.9438469
ACGGTGT12350.08.8651098
TACGGTG13550.08.7442427
CTGTCCG2957.740891E-88.6429979
Produced by FastQC (version 0.11.2)