##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HK5NHBGXX_combined_n02_ntr_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13 Sequences flagged as poor quality 0 Sequence length 36 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0 0.0 0.0 0.0 0.0 0.0 2 32.0 0.0 0.0 0.0 0.0 0.0 3 29.23076923076923 0.0 0.0 0.0 0.0 0.0 4 32.0 0.0 0.0 0.0 0.0 0.0 5 32.0 0.0 0.0 0.0 0.0 0.0 6 36.0 0.0 0.0 0.0 0.0 0.0 7 35.69230769230769 0.0 0.0 0.0 0.0 0.0 8 35.69230769230769 0.0 0.0 0.0 0.0 0.0 9 36.0 0.0 0.0 0.0 0.0 0.0 10 34.0 0.0 0.0 0.0 0.0 0.0 11 34.30769230769231 0.0 0.0 0.0 0.0 0.0 12 34.0 0.0 0.0 0.0 0.0 0.0 13 34.84615384615385 0.0 0.0 0.0 0.0 0.0 14 34.30769230769231 0.0 0.0 0.0 0.0 0.0 15 35.69230769230769 0.0 0.0 0.0 0.0 0.0 16 36.0 0.0 0.0 0.0 0.0 0.0 17 36.0 0.0 0.0 0.0 0.0 0.0 18 34.30769230769231 0.0 0.0 0.0 0.0 0.0 19 36.0 0.0 0.0 0.0 0.0 0.0 20 34.0 0.0 0.0 0.0 0.0 0.0 21 36.0 0.0 0.0 0.0 0.0 0.0 22 34.30769230769231 0.0 0.0 0.0 0.0 0.0 23 34.23076923076923 0.0 0.0 0.0 0.0 0.0 24 36.0 0.0 0.0 0.0 0.0 0.0 25 34.53846153846154 0.0 0.0 0.0 0.0 0.0 26 34.0 0.0 0.0 0.0 0.0 0.0 27 36.0 0.0 0.0 0.0 0.0 0.0 28 34.30769230769231 0.0 0.0 0.0 0.0 0.0 29 35.69230769230769 0.0 0.0 0.0 0.0 0.0 30 30.923076923076923 0.0 0.0 0.0 0.0 0.0 31 35.69230769230769 0.0 0.0 0.0 0.0 0.0 32 35.69230769230769 0.0 0.0 0.0 0.0 0.0 33 35.69230769230769 0.0 0.0 0.0 0.0 0.0 34 36.0 0.0 0.0 0.0 0.0 0.0 35 34.15384615384615 0.0 0.0 0.0 0.0 0.0 36 36.0 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 23612 1 0.0 23612 2 0.0 23612 3 0.0 23612 4 0.0 23612 5 0.0 23612 6 0.0 23612 7 0.0 23612 8 0.0 23612 9 0.0 23612 10 0.0 23612 11 0.0 23612 12 0.0 23612 13 0.0 23612 14 0.0 23612 15 0.0 23612 16 0.0 23612 17 0.0 23612 18 0.0 23612 19 0.0 23612 20 0.0 23612 21 0.0 23612 22 0.0 23612 23 0.0 23612 24 0.0 23612 25 0.0 23612 26 0.0 23612 27 0.0 23612 28 0.0 23612 29 0.0 23612 30 0.0 23612 31 0.0 23612 32 0.0 23612 33 0.0 23612 34 0.0 23612 35 0.0 23612 36 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 32 2.0 33 0.0 34 5.0 35 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.76923076923077 15.384615384615385 23.076923076923077 30.76923076923077 2 23.076923076923077 23.076923076923077 46.15384615384615 7.6923076923076925 3 15.384615384615385 15.384615384615385 38.46153846153847 30.76923076923077 4 7.6923076923076925 7.6923076923076925 46.15384615384615 38.46153846153847 5 15.384615384615385 38.46153846153847 38.46153846153847 7.6923076923076925 6 30.76923076923077 53.84615384615385 15.384615384615385 0.0 7 7.6923076923076925 30.76923076923077 61.53846153846154 0.0 8 23.076923076923077 15.384615384615385 46.15384615384615 15.384615384615385 9 30.76923076923077 7.6923076923076925 46.15384615384615 15.384615384615385 10 23.076923076923077 38.46153846153847 23.076923076923077 15.384615384615385 11 30.76923076923077 38.46153846153847 15.384615384615385 15.384615384615385 12 7.6923076923076925 46.15384615384615 30.76923076923077 15.384615384615385 13 23.076923076923077 30.76923076923077 15.384615384615385 30.76923076923077 14 38.46153846153847 7.6923076923076925 30.76923076923077 23.076923076923077 15 23.076923076923077 23.076923076923077 15.384615384615385 38.46153846153847 16 30.76923076923077 38.46153846153847 15.384615384615385 15.384615384615385 17 23.076923076923077 15.384615384615385 23.076923076923077 38.46153846153847 18 15.384615384615385 38.46153846153847 38.46153846153847 7.6923076923076925 19 7.6923076923076925 23.076923076923077 38.46153846153847 30.76923076923077 20 23.076923076923077 46.15384615384615 7.6923076923076925 23.076923076923077 21 15.384615384615385 23.076923076923077 30.76923076923077 30.76923076923077 22 23.076923076923077 30.76923076923077 38.46153846153847 7.6923076923076925 23 15.384615384615385 30.76923076923077 46.15384615384615 7.6923076923076925 24 15.384615384615385 30.76923076923077 30.76923076923077 23.076923076923077 25 23.076923076923077 46.15384615384615 15.384615384615385 15.384615384615385 26 23.076923076923077 15.384615384615385 30.76923076923077 30.76923076923077 27 38.46153846153847 38.46153846153847 7.6923076923076925 15.384615384615385 28 23.076923076923077 53.84615384615385 15.384615384615385 7.6923076923076925 29 15.384615384615385 15.384615384615385 46.15384615384615 23.076923076923077 30 23.076923076923077 38.46153846153847 30.76923076923077 7.6923076923076925 31 30.76923076923077 0.0 30.76923076923077 38.46153846153847 32 23.076923076923077 38.46153846153847 23.076923076923077 15.384615384615385 33 15.384615384615385 7.6923076923076925 30.76923076923077 46.15384615384615 34 30.76923076923077 23.076923076923077 46.15384615384615 0.0 35 30.76923076923077 23.076923076923077 30.76923076923077 15.384615384615385 36 23.076923076923077 30.76923076923077 38.46153846153847 7.6923076923076925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 1.0 33 2.0 34 2.0 35 2.5 36 3.0 37 3.0 38 2.0 39 1.0 40 2.0 41 3.0 42 3.0 43 2.0 44 1.0 45 1.0 46 1.0 47 1.0 48 1.0 49 1.0 50 1.0 51 0.5 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.5 58 1.0 59 1.0 60 0.5 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 13.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCAATTTAGACCAGTTCAATGGACGATGGGTGTA 1 7.6923076923076925 No Hit GGGAGATCCAAATGCAACAGCAACGCCTGCTGCAGC 1 7.6923076923076925 No Hit CCTTTTATCAGACACGGACAATTTTAAGTGCTTTCT 1 7.6923076923076925 No Hit TGGGAATTGGTTGTTTGGATCGTCAAGGTTGACATT 1 7.6923076923076925 No Hit TACTTGTATTACGGCGGTTATTTCACAAAATACTCA 1 7.6923076923076925 No Hit GACCCAATGTATCTGGCAGAGTAAGGCTTTCCCTTT 1 7.6923076923076925 No Hit CTCTTGTTTGAAAGCATTTATGTTGGAAGACAGAGT 1 7.6923076923076925 No Hit CTTCTATAGACTACCTTACGCATTCTTGCGTTCGGA 1 7.6923076923076925 No Hit ATCCGGGGTCTTCCAACTCCTTAGATGCCATACTAG 1 7.6923076923076925 No Hit ATTTATTGGCACTGTCCATGACTACCAATTCGTTAT 1 7.6923076923076925 No Hit CTATAATTTGGAGGAAATTCTGGATTAACTGAGGAA 1 7.6923076923076925 No Hit TGATAGACAACAATGCCATCCAATATGATAGCAGTG 1 7.6923076923076925 No Hit GATCTAAGTTGGATGACGAAGACAAGGAAACTTTGG 1 7.6923076923076925 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE