FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6716160
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86850.12931496569468268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT35850.010.5846754
GTATTAG18800.010.5338811
CTAATAC21550.010.439833
TAGGTAG9200.010.2705975
GTGTAGG4950.09.698781
TATACTG27450.09.6164175
ACTATGC18250.09.6152268
TCTATAC20200.09.3555353
GTATTAA21600.09.2378351
CCTAATA21150.09.2191082
TTAGGTA16800.09.0169214
GTATAAT32450.08.8768491
GTATAGA22300.08.8133041
GGTAGTC9900.08.7869017
GTATATA60600.08.689741
TAGTCAG9700.08.6587029
GCCCCCG1757.7107095E-48.57105114
ATTAGGT18200.08.5706023
GTGCGCG2454.281599E-68.5703489
GTCTTAG7600.08.4884261
Produced by FastQC (version 0.11.2)