FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5715352
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99140.17346263187289251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8600.012.7307465
GTATTAG16300.010.6772511
GGTAGTC8400.010.5342167
GTATAGA16200.010.0022521
TAGTCAG8050.09.8742859
CTAATAC18350.09.6446573
GGACTGT5100.09.4104256
CTATGCA13250.09.3948079
TAATACT28150.09.377164
ACTATGC15750.09.3318418
CCTAATA19500.09.2300212
TATACTG23050.09.1743995
TATTAGG18300.09.0976232
TACGGTG12550.08.9628687
CTTACAC10250.08.9257943
TACTATG18050.08.8907017
TAGACTG6600.08.8623755
GTATATA56000.08.8412761
TATACGG16000.08.8112465
GTATTAA19200.08.751971
Produced by FastQC (version 0.11.2)