FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7165292
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113620.15856995081289083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21200.011.606931
TAGGTAG10800.010.8318955
CTAATAC21400.09.67167953
CTTACAC9200.09.4553323
TCTATAC21400.09.3913413
GTATTAT38650.09.2780731
ACGGTGT13500.09.2212558
GTATATA68450.09.1843821
ACTATGC19900.09.0442778
GTCTTAT11300.09.0290041
TAATACT34700.08.990164
GTATAGA22700.08.9892291
TAGACTG8350.08.9808435
TATTAGG22150.08.9386762
GTCCTAA7900.08.9264781
GGTAGTC10350.08.8396527
CAGCGTG6800.08.8225429
CCTAGCG4301.8189894E-128.72257130
TACGGTG15150.08.7119577
CTAGGAC4650.08.7085813
Produced by FastQC (version 0.11.2)