FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4875789
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85570.17549980116038655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC7550.016.487767
TAGGTAG8200.016.4609435
TAGTCAG7750.014.7074719
CGATCAC4300.013.25831124
GTATTAG16400.013.1733481
CCCGGGG1101.04560204E-412.2710117
TTAGGTA12750.011.880574
CTAATAC16050.011.5871653
CCTAGCG4450.011.46342230
CTATGCA12300.011.3397639
TATTAGG15950.011.2841512
ATCACAC10250.011.124845529
GTAGTCA11250.010.7983798
ACTATGC16300.010.7652568
TGGCGAC4750.010.73941530
TACTATG18400.010.4333247
ATTAGGT15050.010.3643
ACCTAAT15850.010.3175071
CTAGGCA5300.010.18725510
AGTCAGA11700.010.12678910
Produced by FastQC (version 0.11.2)