FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332318
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56980.24430630814494422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG3300.019.5432745
GGTAGTC3400.014.55727
TAGTCAG3150.014.2841219
GTATTAG5850.013.8490631
TATTAGG6650.012.6307122
TTAGGTA5700.012.3673084
CTTACAC2257.2759576E-1111.99891953
ATTAGGT5650.011.4149023
CGTGAGC2659.094947E-1211.31973612
GCCCGAA1202.3480378E-411.24898711
ACGTGAG2957.2759576E-1210.67700411
CTAGGAC1551.9582181E-510.6442023
CTAATAC6200.010.4022883
GTATAGA6000.010.2521541
TAGGACC2209.052383E-810.2263524
GTAGTCA4850.010.2050488
TATACCG4850.09.8958035
TATTATA10800.09.8604352
GGACCAT2905.693437E-109.826496
GTATTAA5650.09.8250731
Produced by FastQC (version 0.11.2)