FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528546
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101960.40323569355669225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG1900.015.78922516
CTATGCA5500.014.4526019
GCGCACT3100.013.54683211
CACTCCG2250.013.33180414
TAATACT12200.013.0312914
GTCGGGG2350.012.76474515
ATCACAC5350.012.61911929
ACTATGC8550.012.4544668
CGGGGGC2650.012.4528817
ACCTAAT6550.012.3713551
CCTCGCG1354.2197407E-612.2205799
CCTAATA6550.012.1369722
TACTATG7850.012.0365977
GGGTTAC2257.094059E-1112.0047961
CTAACCG1006.460985E-411.9983869
ATATGAC7100.011.8298633
CGGTCGG2553.6379788E-1211.7635913
CCCGGGG1151.5818133E-411.7375527
TCACACA5950.011.59874930
TAGACTG2103.754394E-911.4270345
Produced by FastQC (version 0.11.2)