FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6360066
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93730.14737268449729923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10650.014.5056475
GTATTAG19600.013.9328181
GGTAGTC10250.013.9011027
TAGTCAG11150.012.779049
CCCCGGG900.004011969511.66609512
TATTAGG20050.011.5954432
CTATGCA16450.011.4882579
ACTATGC21000.010.7131718
CCTAATA21050.010.6170572
TTAGGTA16600.010.480924
CTAATAC23550.010.3175613
GTATAGA19350.010.2356791
CCTAGCG4700.010.21427330
ACGGTGT12650.010.1965678
ATTAGGT19050.010.0778493
TAATACT35000.010.070464
ACCTAAT20950.09.9553021
TAGACTG5750.09.912095
ATCACAC15200.09.87003329
TCGCCCG4000.09.75067124
Produced by FastQC (version 0.11.2)