FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000376
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55410.18467685383431945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4800.014.0606565
GTATTAG7900.013.8635631
TTAGGTA7000.011.9984264
CTAATAC9900.011.2106513
TAATACT15150.011.0876554
TATTAGG8650.010.9243342
GGTAGTC4500.010.6654467
TCTATAC8550.010.5249363
ACTATGC8900.010.4484168
TAGTCAG4450.010.111549
CTATGCA8050.010.0612969
ATCACAC5850.010.00168929
TCGGGGG1501.64522E-49.99985616
AATACTA12600.09.9986885
CTGATCG2003.0491683E-69.7492099
CTTAGGC2003.0511146E-69.7487223
GTATAGA9100.09.5623731
CCTAATA9900.09.5449992
ATTAGGT8350.09.519713
TACTATG9800.09.3356677
Produced by FastQC (version 0.11.2)