FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503957
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10810.21450242778649764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA652.7859642E-823.0712749
GGACATG402.4606494E-422.5056571
CTGAGGG706.106529E-821.4233269
GTCTTAG455.451462E-420.005031
TAAGCCT751.2652345E-719.9951044
TTAGAAC752.793744E-617.9973813
ATACCCT500.001110870517.9955944
GCTATCC703.1107018E-517.1403623
TCGGCAG805.163767E-616.87421821
TCTCAAC550.002101483316.3612543
TACGTTC653.4356353E-416.15149510
TAGGTAG653.4382337E-416.1498935
GTATAAC755.5987595E-516.0040251
TACCCTT755.622579E-515.9960835
GGATATG859.160873E-615.8863471
CCTAAGG859.195455E-615.8800413
TATGACA859.204119E-615.8784654
AAGCCTT1052.476736E-715.710445
CATGGGT600.003743755415.00377325
GTCTAAA901.5743019E-515.0037721
Produced by FastQC (version 0.11.2)