FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362273
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31000.22756084866983342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG651.6467415E-518.4609287
TAGGTAG2450.018.3660665
GTATTAG3600.016.6703991
GGCCCCC706.039388E-414.99950511
GGACCGC600.003756138514.9989546
GGTAGTC2600.014.42267
CGATCAC1802.1827873E-1114.166718524
ACCGCGC851.6342537E-414.1171818
CCCTAAG750.001016393314.0031361
TAGTCAG2250.013.9995389
GGCCGAG750.001018369713.99953711
GAGACTG2150.013.9525165
ACAGTCC1454.7402864E-813.4478328
AGACTAG1905.4569682E-1113.4206097
CTAGGCA1905.4569682E-1113.42060910
GGACTAG902.647486E-413.3363191
TAGGGGT800.001657551713.1240854
TACACAG2500.012.5991215
GACTGTT2401.8189894E-1212.4995877
TAAGACA3000.012.4991284
Produced by FastQC (version 0.11.2)