FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length36
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGTACTAGTTCTCTTTTAAGAATTTCCAATTC15.0No Hit
GAGTGAAAAATTAAAATAAAAAAAGGGCCAAAAAAA15.0No Hit
CCTACGGCATTCGGAACCTGATGCATTCCAAAGCAA15.0No Hit
ATTTTGTTTGAAGAAGTTGGCTCCTCATAGGTGTTA15.0No Hit
GTAATCTACTGTGTCGGAATAGAAAGTCATAAATGT15.0No Hit
AGAATGAAGAATGCGCTGAGTAAGCTTTCCATCACT15.0No Hit
GCATAATAGTATCCTGATAGGAGGGTTCCTTAGGTA15.0No Hit
GAGTGAAATTAATTCCTATTACCAAGAACGCCATAT15.0No Hit
CTCAGAAGCAGTCTGAATCTCTCGGATCAATTATCT15.0No Hit
TACATATGTTAACTGGTGACCACCCTGGTACTGCTA15.0No Hit
GTATTGGTGGATCTTCTTTCTTTCTTCTCTTGGAAT15.0No Hit
GTAAATCGTTTAATCTATCTGTTAATGCGTTTCCGA15.0No Hit
CGTAAAAAGTCGCCAAAAATTAGGGAAGAATAAGGC15.0No Hit
TAATGAATGCTTGGATGTGCTAGCGTGGGGTGTTTG15.0No Hit
GGGTAATAGAGGAAGAACTGTACAATAAAAACTGAG15.0No Hit
CAATGAACGCATTGCCTGTAACCTAGCTGCTCCGAG15.0No Hit
CATCGGGAACTGGATCAGCCTCTGAAACCGGAAGCT15.0No Hit
GTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTC15.0No Hit
AATCACTTGTGATGCCCTCTCAACGATCCTACACCC15.0No Hit
CCCTACCACTCTAATCCCACTACCACATGCCATAGC15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)