##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HK5NHBGXX_combined_n02_ntr_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20 Sequences flagged as poor quality 0 Sequence length 36 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0 0.0 0.0 0.0 0.0 0.0 2 32.0 0.0 0.0 0.0 0.0 0.0 3 32.0 0.0 0.0 0.0 0.0 0.0 4 32.0 0.0 0.0 0.0 0.0 0.0 5 29.65 0.0 0.0 0.0 0.0 0.0 6 35.8 0.0 0.0 0.0 0.0 0.0 7 35.6 0.0 0.0 0.0 0.0 0.0 8 35.6 0.0 0.0 0.0 0.0 0.0 9 36.0 0.0 0.0 0.0 0.0 0.0 10 34.9 0.0 0.0 0.0 0.0 0.0 11 36.0 0.0 0.0 0.0 0.0 0.0 12 34.7 0.0 0.0 0.0 0.0 0.0 13 34.7 0.0 0.0 0.0 0.0 0.0 14 34.5 0.0 0.0 0.0 0.0 0.0 15 35.8 0.0 0.0 0.0 0.0 0.0 16 34.9 0.0 0.0 0.0 0.0 0.0 17 34.3 0.0 0.0 0.0 0.0 0.0 18 35.4 0.0 0.0 0.0 0.0 0.0 19 33.8 0.0 0.0 0.0 0.0 0.0 20 35.8 0.0 0.0 0.0 0.0 0.0 21 34.7 0.0 0.0 0.0 0.0 0.0 22 35.6 0.0 0.0 0.0 0.0 0.0 23 34.9 0.0 0.0 0.0 0.0 0.0 24 36.0 0.0 0.0 0.0 0.0 0.0 25 35.35 0.0 0.0 0.0 0.0 0.0 26 34.9 0.0 0.0 0.0 0.0 0.0 27 35.15 0.0 0.0 0.0 0.0 0.0 28 34.9 0.0 0.0 0.0 0.0 0.0 29 36.0 0.0 0.0 0.0 0.0 0.0 30 33.6 0.0 0.0 0.0 0.0 0.0 31 35.35 0.0 0.0 0.0 0.0 0.0 32 33.95 0.0 0.0 0.0 0.0 0.0 33 34.9 0.0 0.0 0.0 0.0 0.0 34 36.0 0.0 0.0 0.0 0.0 0.0 35 36.0 0.0 0.0 0.0 0.0 0.0 36 34.1 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 22602 1 0.0 22602 2 0.0 22602 3 0.0 22602 4 0.0 22602 5 0.0 22602 6 0.0 22602 7 0.0 22602 8 0.0 22602 9 0.0 22602 10 0.0 22602 11 0.0 22602 12 0.0 22602 13 0.0 22602 14 0.0 22602 15 2.0 22602 16 0.0 22602 17 0.0 22602 18 0.0 22602 19 0.0 22602 20 0.0 22602 21 0.0 22602 22 0.0 22602 23 0.0 22602 24 0.0 22602 25 0.0 22602 26 0.0 22602 27 0.0 22602 28 0.0 22602 29 0.0 22602 30 11.0 22602 31 0.0 22602 32 0.0 22602 33 0.0 22602 34 0.0 22602 35 0.0 22602 36 -2.0 12408 1 0.0 12408 2 0.0 12408 3 0.0 12408 4 0.0 12408 5 0.0 12408 6 0.0 12408 7 0.0 12408 8 0.0 12408 9 0.0 12408 10 0.0 12408 11 0.0 12408 12 0.0 12408 13 0.0 12408 14 0.0 12408 15 -2.0 12408 16 0.0 12408 17 0.0 12408 18 0.0 12408 19 0.0 12408 20 0.0 12408 21 0.0 12408 22 0.0 12408 23 0.0 12408 24 0.0 12408 25 0.0 12408 26 0.0 12408 27 0.0 12408 28 0.0 12408 29 0.0 12408 30 -11.0 12408 31 0.0 12408 32 0.0 12408 33 0.0 12408 34 0.0 12408 35 0.0 12408 36 2.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 32 3.0 33 0.0 34 8.0 35 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.0 15.0 10.0 35.0 2 15.0 40.0 30.0 15.0 3 15.0 40.0 25.0 20.0 4 0.0 40.0 50.0 10.0 5 30.0 40.0 25.0 5.0 6 30.0 50.0 5.0 15.0 7 15.0 35.0 35.0 15.0 8 15.0 50.0 25.0 10.0 9 40.0 20.0 20.0 20.0 10 15.0 20.0 50.0 15.0 11 20.0 45.0 25.0 10.0 12 25.0 25.0 45.0 5.0 13 30.0 20.0 25.0 25.0 14 20.0 25.0 35.0 20.0 15 20.0 30.0 20.0 30.0 16 20.0 30.0 15.0 35.0 17 15.0 30.0 35.0 20.0 18 20.0 20.0 40.0 20.0 19 10.0 35.0 35.0 20.0 20 25.0 10.0 35.0 30.0 21 10.0 30.0 40.0 20.0 22 5.0 55.00000000000001 25.0 15.0 23 20.0 25.0 15.0 40.0 24 30.0 30.0 5.0 35.0 25 35.0 45.0 10.0 10.0 26 25.0 25.0 40.0 10.0 27 25.0 30.0 35.0 10.0 28 15.0 10.0 35.0 40.0 29 20.0 25.0 10.0 45.0 30 20.0 30.0 30.0 20.0 31 10.0 30.0 40.0 20.0 32 10.0 40.0 25.0 25.0 33 25.0 40.0 20.0 15.0 34 25.0 20.0 35.0 20.0 35 20.0 30.0 30.0 20.0 36 15.0 30.0 30.0 25.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.0 23 1.0 24 0.5 25 0.0 26 0.5 27 1.0 28 1.0 29 0.5 30 0.0 31 0.0 32 1.5 33 3.0 34 3.0 35 3.0 36 3.0 37 3.0 38 2.0 39 1.0 40 2.0 41 3.0 42 3.0 43 2.5 44 2.0 45 2.0 46 1.5 47 1.0 48 1.0 49 1.5 50 2.0 51 2.0 52 2.0 53 2.0 54 1.5 55 1.0 56 1.0 57 0.5 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 20.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CAATAGTACTAGTTCTCTTTTAAGAATTTCCAATTC 1 5.0 No Hit GAGTGAAAAATTAAAATAAAAAAAGGGCCAAAAAAA 1 5.0 No Hit CCTACGGCATTCGGAACCTGATGCATTCCAAAGCAA 1 5.0 No Hit ATTTTGTTTGAAGAAGTTGGCTCCTCATAGGTGTTA 1 5.0 No Hit GTAATCTACTGTGTCGGAATAGAAAGTCATAAATGT 1 5.0 No Hit AGAATGAAGAATGCGCTGAGTAAGCTTTCCATCACT 1 5.0 No Hit GCATAATAGTATCCTGATAGGAGGGTTCCTTAGGTA 1 5.0 No Hit GAGTGAAATTAATTCCTATTACCAAGAACGCCATAT 1 5.0 No Hit CTCAGAAGCAGTCTGAATCTCTCGGATCAATTATCT 1 5.0 No Hit TACATATGTTAACTGGTGACCACCCTGGTACTGCTA 1 5.0 No Hit GTATTGGTGGATCTTCTTTCTTTCTTCTCTTGGAAT 1 5.0 No Hit GTAAATCGTTTAATCTATCTGTTAATGCGTTTCCGA 1 5.0 No Hit CGTAAAAAGTCGCCAAAAATTAGGGAAGAATAAGGC 1 5.0 No Hit TAATGAATGCTTGGATGTGCTAGCGTGGGGTGTTTG 1 5.0 No Hit GGGTAATAGAGGAAGAACTGTACAATAAAAACTGAG 1 5.0 No Hit CAATGAACGCATTGCCTGTAACCTAGCTGCTCCGAG 1 5.0 No Hit CATCGGGAACTGGATCAGCCTCTGAAACCGGAAGCT 1 5.0 No Hit GTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTC 1 5.0 No Hit AATCACTTGTGATGCCCTCTCAACGATCCTACACCC 1 5.0 No Hit CCCTACCACTCTAATCCCACTACCACATGCCATAGC 1 5.0 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE