FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6744342
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112920.16742923179162622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG12800.014.5301665
GTATTAG20000.013.8029611
TAGTCAG11850.013.6699679
GGTAGTC12100.013.5113877
CTAATAC23350.012.7826783
TATTAGG20850.012.6613952
TTAGGTA16750.012.4468334
CCTAGCG7200.012.08413330
CGATCAC7500.011.80069424
CTATGCA19600.011.4022959
TAATACT36450.011.3571774
CCTAATA23150.011.2738742
ACCTAAT21350.011.2436291
AATACTA28100.010.8888675
ATCACAC16950.010.708673529
ATTAGGT20050.010.622573
TCACACA17550.010.51351630
GTCCTAA9250.010.3806051
GTATAGA23000.010.371791
ACTATGC26250.010.2849468
Produced by FastQC (version 0.11.2)