FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8026301
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149520.18628755637248093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG16250.015.13645655
TAGTCAG14100.015.1046649
GGTAGTC14750.014.9472647
GTATTAG26250.014.1741431
CCTAGCG7500.013.00240830
TATTAGG24650.012.4740032
TTAGGTA21450.012.23621754
ATTAGGT24750.011.1503153
CTATGCA20900.011.0513749
GTATAGA24900.011.0262081
CGATCAC8500.010.94245424
AGTCAGA20850.010.79020610
GTAGTCA21750.010.6882398
CTAATAC26900.010.5936563
TAATACT38450.010.531834
TCACACA19200.010.3925530
CACCTAG9400.010.21459528
ACTATGC26200.010.0750018
ATCACAC17750.010.05838929
ACCTAGC9250.010.05610529
Produced by FastQC (version 0.11.2)