FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6032502
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78750.1305428493848821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10600.015.2814445
GTATTAG18800.014.7627881
GGTAGTC10450.014.639767
TAGTCAG10250.014.4864329
CTATGCA14800.013.2757699
TTAGGTA17200.012.3824444
CTAATAC21900.011.9852653
ACTATGC21100.011.79982958
TATTAGG20350.011.7927872
ATCACAC15550.011.57724929
CTAGGAC3950.011.3914193
TCACACA16100.010.99566430
AGTCAGA14350.010.9745710
CCTAATA23550.010.9546482
ATTAGGT19350.010.9292873
GTCAGAT15300.010.58749511
GTAGTCA14900.010.4688068
TACTATG24550.010.4470747
AGGTAGT17500.010.3704466
ACCTAAT21900.010.3439721
Produced by FastQC (version 0.11.2)