FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6708617
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116980.17437275074728517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG10200.013.3807219
TAGGTAG11450.013.3609155
GTATTAG20600.012.7450581
GGTAGTC11600.012.2830767
CTATGCA16850.011.7493099
ACTATGC20950.011.59763058
ATCACAC14150.011.23872929
GCCGCGT5000.011.101624525
CTAATAC25950.011.0391983
TATTAGG21250.010.5169922
TAATACT35300.010.4520614
GACGCGC2905.2750693E-1110.3435668
CTAGCCT5100.010.2929384
CGCCGCG5450.010.18429324
TTAGGTA18250.010.1084314
CCTAATA23450.010.1059792
CCTAGCG4800.09.68913530
TATACTG26750.09.6437565
AGTCAGA15400.09.64168210
ACCTAAT23600.09.599221
Produced by FastQC (version 0.11.2)