FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6808953
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104850.15398843258280678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG11450.012.3130755
CTAATAC23400.011.15255453
TAGTCAG10200.011.0282969
GTATTAG19550.010.5898871
ACTATGC20450.010.5612798
GGTAGTC11600.010.4730797
TATACTG27550.010.1259435
TCTATAC18800.09.972253
TTAGGTA18350.09.88993554
TATTAGG22350.09.6633822
ATCACAC13300.09.5876829
CTATGCA17550.09.5716829
TAATACT37900.09.2602094
ACGGTGT14100.09.2543838
ACTGTGC9900.09.089998
TACTATG25200.08.8681517
TCACACA15750.08.85832930
TAGACTG6650.08.7960395
GTATATA68250.08.7046541
CCTAATA24750.08.6657272
Produced by FastQC (version 0.11.2)