FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6221661
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104690.16826696279337625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG16550.011.0600211
TTATACT23850.09.9362654
CTGAGCG4250.09.8815969
TAGGTAG8350.09.8794035
TATACTG21050.09.6903925
TGCGACG5500.09.54664230
TATACAG29650.09.35839755
GTCTTAG7700.09.3528641
CGCTCGC1450.0013400389.31030521
GTATATA51100.09.1900431
TCGGTCG2157.653716E-69.0690739
GTATAAT28050.09.0395741
TATTAGG17450.08.9392872
CTAATAC16050.08.7842333
ACTGCCC5500.08.7266048
TAATACT29450.08.70894054
GTATATG28150.08.6343721
GTATTAA19500.08.6174251
GTATAGA18300.08.6085971
ACTATGC15200.08.5848688
Produced by FastQC (version 0.11.2)