FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7431758
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131580.17705097501829312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1501.042632E-611.998949
GTATTAG21900.011.6463341
GGTAGTC9400.011.0094437
CTAATAC20850.010.9339913
ACTATGC17500.010.2845978
TAGGTAG13350.010.2235415
TAATACT33650.09.671994
CTATGCA16650.09.4586249
TATTAGG22250.09.3702742
GTATAGA24100.09.0890871
CCTAGCG5450.09.08408830
CCTACAC7200.08.9573013
CCTAATA23550.08.7256372
TATACTG26350.08.70867355
TATTATA59600.08.6824112
GTATTAA24600.08.5994041
GTATATG27050.08.5970371
ATTAGGT18250.08.4647773
GTATATA58050.08.4514251
GTATAAT32700.08.39629651
Produced by FastQC (version 0.11.2)