##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	HK5NHBGXX_combined_n02_ntr_11.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27
Sequences flagged as poor quality	0
Sequence length	36
%GC	36
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.333333333333332	0.0	0.0	0.0	0.0	0.0
2	30.925925925925927	0.0	0.0	0.0	0.0	0.0
3	31.333333333333332	0.0	0.0	0.0	0.0	0.0
4	30.666666666666668	0.0	0.0	0.0	0.0	0.0
5	30.666666666666668	0.0	0.0	0.0	0.0	0.0
6	33.51851851851852	0.0	0.0	0.0	0.0	0.0
7	34.629629629629626	0.0	0.0	0.0	0.0	0.0
8	33.74074074074074	0.0	0.0	0.0	0.0	0.0
9	35.111111111111114	0.0	0.0	0.0	0.0	0.0
10	32.74074074074074	0.0	0.0	0.0	0.0	0.0
11	34.7037037037037	0.0	0.0	0.0	0.0	0.0
12	31.88888888888889	0.0	0.0	0.0	0.0	0.0
13	34.074074074074076	0.0	0.0	0.0	0.0	0.0
14	33.25925925925926	0.0	0.0	0.0	0.0	0.0
15	34.370370370370374	0.0	0.0	0.0	0.0	0.0
16	34.7037037037037	0.0	0.0	0.0	0.0	0.0
17	34.55555555555556	0.0	0.0	0.0	0.0	0.0
18	34.81481481481482	0.0	0.0	0.0	0.0	0.0
19	33.925925925925924	0.0	0.0	0.0	0.0	0.0
20	34.22222222222222	0.0	0.0	0.0	0.0	0.0
21	33.96296296296296	0.0	0.0	0.0	0.0	0.0
22	34.333333333333336	0.0	0.0	0.0	0.0	0.0
23	31.333333333333332	0.0	0.0	0.0	0.0	0.0
24	32.925925925925924	0.0	0.0	0.0	0.0	0.0
25	34.18518518518518	0.0	0.0	0.0	0.0	0.0
26	32.7037037037037	0.0	0.0	0.0	0.0	0.0
27	34.074074074074076	0.0	0.0	0.0	0.0	0.0
28	34.74074074074074	0.0	0.0	0.0	0.0	0.0
29	33.55555555555556	0.0	0.0	0.0	0.0	0.0
30	31.77777777777778	0.0	0.0	0.0	0.0	0.0
31	33.592592592592595	0.0	0.0	0.0	0.0	0.0
32	32.592592592592595	0.0	0.0	0.0	0.0	0.0
33	32.77777777777778	0.0	0.0	0.0	0.0	0.0
34	34.592592592592595	0.0	0.0	0.0	0.0	0.0
35	35.22222222222222	0.0	0.0	0.0	0.0	0.0
36	33.55555555555556	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
12508	1	0.0
12508	2	0.0
12508	3	0.0
12508	4	0.0
12508	5	0.0
12508	6	2.0
12508	7	0.0
12508	8	0.0
12508	9	0.0
12508	10	0.0
12508	11	0.0
12508	12	11.0
12508	13	2.0
12508	14	2.0
12508	15	11.0
12508	16	0.0
12508	17	0.0
12508	18	2.0
12508	19	11.0
12508	20	0.0
12508	21	2.0
12508	22	0.0
12508	23	0.0
12508	24	0.0
12508	25	-2.0
12508	26	-2.0
12508	27	0.0
12508	28	2.0
12508	29	0.0
12508	30	-11.0
12508	31	4.5
12508	32	0.0
12508	33	11.0
12508	34	-2.5
12508	35	-2.0
12508	36	0.0
12210	1	0.0
12210	2	0.0
12210	3	0.0
12210	4	0.0
12210	5	0.0
12210	6	-2.0
12210	7	0.0
12210	8	0.0
12210	9	0.0
12210	10	0.0
12210	11	0.0
12210	12	-11.0
12210	13	-2.0
12210	14	-2.0
12210	15	-11.0
12210	16	0.0
12210	17	0.0
12210	18	-2.0
12210	19	-11.0
12210	20	0.0
12210	21	-2.0
12210	22	0.0
12210	23	0.0
12210	24	0.0
12210	25	2.0
12210	26	2.0
12210	27	0.0
12210	28	-2.0
12210	29	0.0
12210	30	11.0
12210	31	-4.5
12210	32	0.0
12210	33	-11.0
12210	34	2.5
12210	35	2.0
12210	36	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	2.0
31	3.0
32	4.0
33	3.0
34	7.0
35	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.03703703703704	25.925925925925924	14.814814814814813	22.22222222222222
2	14.814814814814813	29.629629629629626	51.85185185185185	3.7037037037037033
3	18.51851851851852	33.33333333333333	37.03703703703704	11.11111111111111
4	14.814814814814813	18.51851851851852	55.55555555555556	11.11111111111111
5	14.814814814814813	55.55555555555556	22.22222222222222	7.4074074074074066
6	22.22222222222222	37.03703703703704	29.629629629629626	11.11111111111111
7	14.814814814814813	33.33333333333333	29.629629629629626	22.22222222222222
8	18.51851851851852	44.44444444444444	11.11111111111111	25.925925925925924
9	25.925925925925924	14.814814814814813	29.629629629629626	29.629629629629626
10	7.4074074074074066	40.74074074074074	37.03703703703704	14.814814814814813
11	22.22222222222222	40.74074074074074	22.22222222222222	14.814814814814813
12	14.814814814814813	51.85185185185185	22.22222222222222	11.11111111111111
13	18.51851851851852	22.22222222222222	33.33333333333333	25.925925925925924
14	7.4074074074074066	25.925925925925924	25.925925925925924	40.74074074074074
15	25.925925925925924	40.74074074074074	25.925925925925924	7.4074074074074066
16	14.814814814814813	48.148148148148145	25.925925925925924	11.11111111111111
17	22.22222222222222	48.148148148148145	22.22222222222222	7.4074074074074066
18	22.22222222222222	33.33333333333333	25.925925925925924	18.51851851851852
19	11.11111111111111	44.44444444444444	33.33333333333333	11.11111111111111
20	22.22222222222222	40.74074074074074	22.22222222222222	14.814814814814813
21	18.51851851851852	44.44444444444444	18.51851851851852	18.51851851851852
22	33.33333333333333	29.629629629629626	25.925925925925924	11.11111111111111
23	22.22222222222222	40.74074074074074	22.22222222222222	14.814814814814813
24	7.4074074074074066	22.22222222222222	29.629629629629626	40.74074074074074
25	11.11111111111111	40.74074074074074	22.22222222222222	25.925925925925924
26	11.11111111111111	48.148148148148145	25.925925925925924	14.814814814814813
27	11.11111111111111	48.148148148148145	25.925925925925924	14.814814814814813
28	22.22222222222222	18.51851851851852	25.925925925925924	33.33333333333333
29	25.925925925925924	40.74074074074074	18.51851851851852	14.814814814814813
30	7.4074074074074066	44.44444444444444	29.629629629629626	18.51851851851852
31	7.4074074074074066	29.629629629629626	25.925925925925924	37.03703703703704
32	18.51851851851852	25.925925925925924	44.44444444444444	11.11111111111111
33	37.03703703703704	14.814814814814813	29.629629629629626	18.51851851851852
34	11.11111111111111	37.03703703703704	22.22222222222222	29.629629629629626
35	22.22222222222222	33.33333333333333	33.33333333333333	11.11111111111111
36	14.814814814814813	55.55555555555556	18.51851851851852	11.11111111111111
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.0
18	1.0
19	1.0
20	1.0
21	1.0
22	1.0
23	1.0
24	1.5
25	2.0
26	2.5
27	3.0
28	3.0
29	2.0
30	1.0
31	1.0
32	1.5
33	2.0
34	2.0
35	3.0
36	4.0
37	4.0
38	4.0
39	4.0
40	3.0
41	2.0
42	2.0
43	2.0
44	2.0
45	2.0
46	1.5
47	1.0
48	1.0
49	1.0
50	1.0
51	0.5
52	0.0
53	0.0
54	0.5
55	1.0
56	1.0
57	0.5
58	0.0
59	0.0
60	0.5
61	1.0
62	1.0
63	0.5
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	27.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	100.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	100.0	100.0
2	0.0	0.0
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATGTACTGTTGCCATAGTAACGTAAATTATGTAAA	1	3.7037037037037033	No Hit
CTTAACCCTCGCTTTGTTGCTGAGCCAGAACTCTTT	1	3.7037037037037033	No Hit
GAGTAGTCCCGTGGGAAGAGCTACTAGCCGAAGCGG	1	3.7037037037037033	No Hit
CCTCTCCTCAACCTGCTTCAGTACCACCTCCACAGA	1	3.7037037037037033	No Hit
ATATAGGATTCAATTAGATAATAGAACAAAAGTAAA	1	3.7037037037037033	No Hit
GTGCATGCGTTATCCTATAAAGAAATACGTCCGGAT	1	3.7037037037037033	No Hit
GTGTATCATAAAGAAAACGTTACTATTATTGTCTTA	1	3.7037037037037033	No Hit
GTTTATAAGGGACCAAAAAAAGGTTGGTATATTTTC	1	3.7037037037037033	No Hit
ATATCATATTCATGCTGCAACCTCAATGGTTCACTA	1	3.7037037037037033	No Hit
GAGCAATGCTAACAAGGCTTGGAACAAAGTTTTCCA	1	3.7037037037037033	No Hit
GGATGCAAAAAAACAAATCTCAAACAGGAACTTCTG	1	3.7037037037037033	No Hit
GTAGTAAAGGAACTATCAAATAAACGATAACTGATT	1	3.7037037037037033	No Hit
AGCATATGGAATCCGGAGGTTATTGATCCAAAACAA	1	3.7037037037037033	No Hit
CAATAAAGTAGCTCTTAGTTGAACTGTTGTCAGAAA	1	3.7037037037037033	No Hit
GTGTAATGCTAAGAAGAAAGAGGAGCAGGCACAGTA	1	3.7037037037037033	No Hit
GTATTGTAGATGACGCTGTAAACTGCAATACTTCCA	1	3.7037037037037033	No Hit
ATCACGCACAAAAAAAAAAAAATTAAATAACAAAAA	1	3.7037037037037033	No Hit
CTATATACTACGTATAGGAAATGTTTACATTTTCGT	1	3.7037037037037033	No Hit
TATGAGACTATATCATGACCATACCTTATTTGGAGG	1	3.7037037037037033	No Hit
ATAAATGAGCTTGCGAACTGAGCCATTTACCGGTCA	1	3.7037037037037033	No Hit
AAATGAACCTAGGATTGAATACGCATCCAAAACTGC	1	3.7037037037037033	No Hit
TGTTGGCAATAAATGAAATGGGGCAAATGCTTGGAA	1	3.7037037037037033	No Hit
TTTTGTGTACGTTTTCTTAGCTTCAAACCAATCCAA	1	3.7037037037037033	No Hit
CACAAATAAACATCTAACACTTAATAACAATAGAAA	1	3.7037037037037033	No Hit
ATTTATAGCTGTAAAACTTGCGCTTCCCAGCTTATT	1	3.7037037037037033	No Hit
CATGTATATATATTAATTAAAATCATAGCCATGTGG	1	3.7037037037037033	No Hit
GGTTATACCTATCCGATATCGGTCCATGGAGGGATC	1	3.7037037037037033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE