FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length36
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTACTGTTGCCATAGTAACGTAAATTATGTAAA13.7037037037037033No Hit
CTTAACCCTCGCTTTGTTGCTGAGCCAGAACTCTTT13.7037037037037033No Hit
GAGTAGTCCCGTGGGAAGAGCTACTAGCCGAAGCGG13.7037037037037033No Hit
CCTCTCCTCAACCTGCTTCAGTACCACCTCCACAGA13.7037037037037033No Hit
ATATAGGATTCAATTAGATAATAGAACAAAAGTAAA13.7037037037037033No Hit
GTGCATGCGTTATCCTATAAAGAAATACGTCCGGAT13.7037037037037033No Hit
GTGTATCATAAAGAAAACGTTACTATTATTGTCTTA13.7037037037037033No Hit
GTTTATAAGGGACCAAAAAAAGGTTGGTATATTTTC13.7037037037037033No Hit
ATATCATATTCATGCTGCAACCTCAATGGTTCACTA13.7037037037037033No Hit
GAGCAATGCTAACAAGGCTTGGAACAAAGTTTTCCA13.7037037037037033No Hit
GGATGCAAAAAAACAAATCTCAAACAGGAACTTCTG13.7037037037037033No Hit
GTAGTAAAGGAACTATCAAATAAACGATAACTGATT13.7037037037037033No Hit
AGCATATGGAATCCGGAGGTTATTGATCCAAAACAA13.7037037037037033No Hit
CAATAAAGTAGCTCTTAGTTGAACTGTTGTCAGAAA13.7037037037037033No Hit
GTGTAATGCTAAGAAGAAAGAGGAGCAGGCACAGTA13.7037037037037033No Hit
GTATTGTAGATGACGCTGTAAACTGCAATACTTCCA13.7037037037037033No Hit
ATCACGCACAAAAAAAAAAAAATTAAATAACAAAAA13.7037037037037033No Hit
CTATATACTACGTATAGGAAATGTTTACATTTTCGT13.7037037037037033No Hit
TATGAGACTATATCATGACCATACCTTATTTGGAGG13.7037037037037033No Hit
ATAAATGAGCTTGCGAACTGAGCCATTTACCGGTCA13.7037037037037033No Hit
AAATGAACCTAGGATTGAATACGCATCCAAAACTGC13.7037037037037033No Hit
TGTTGGCAATAAATGAAATGGGGCAAATGCTTGGAA13.7037037037037033No Hit
TTTTGTGTACGTTTTCTTAGCTTCAAACCAATCCAA13.7037037037037033No Hit
CACAAATAAACATCTAACACTTAATAACAATAGAAA13.7037037037037033No Hit
ATTTATAGCTGTAAAACTTGCGCTTCCCAGCTTATT13.7037037037037033No Hit
CATGTATATATATTAATTAAAATCATAGCCATGTGG13.7037037037037033No Hit
GGTTATACCTATCCGATATCGGTCCATGGAGGGATC13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)