##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HK5NHBGXX_combined_n02_ntr_10.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7 Sequences flagged as poor quality 0 Sequence length 36 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0 0.0 0.0 0.0 0.0 0.0 2 29.428571428571427 0.0 0.0 0.0 0.0 0.0 3 29.428571428571427 0.0 0.0 0.0 0.0 0.0 4 29.428571428571427 0.0 0.0 0.0 0.0 0.0 5 32.0 0.0 0.0 0.0 0.0 0.0 6 35.42857142857143 0.0 0.0 0.0 0.0 0.0 7 36.0 0.0 0.0 0.0 0.0 0.0 8 32.285714285714285 0.0 0.0 0.0 0.0 0.0 9 36.0 0.0 0.0 0.0 0.0 0.0 10 35.42857142857143 0.0 0.0 0.0 0.0 0.0 11 32.857142857142854 0.0 0.0 0.0 0.0 0.0 12 32.285714285714285 0.0 0.0 0.0 0.0 0.0 13 32.285714285714285 0.0 0.0 0.0 0.0 0.0 14 32.857142857142854 0.0 0.0 0.0 0.0 0.0 15 31.0 0.0 0.0 0.0 0.0 0.0 16 32.714285714285715 0.0 0.0 0.0 0.0 0.0 17 32.857142857142854 0.0 0.0 0.0 0.0 0.0 18 35.42857142857143 0.0 0.0 0.0 0.0 0.0 19 32.857142857142854 0.0 0.0 0.0 0.0 0.0 20 32.285714285714285 0.0 0.0 0.0 0.0 0.0 21 35.42857142857143 0.0 0.0 0.0 0.0 0.0 22 36.0 0.0 0.0 0.0 0.0 0.0 23 36.0 0.0 0.0 0.0 0.0 0.0 24 34.857142857142854 0.0 0.0 0.0 0.0 0.0 25 36.0 0.0 0.0 0.0 0.0 0.0 26 32.285714285714285 0.0 0.0 0.0 0.0 0.0 27 35.42857142857143 0.0 0.0 0.0 0.0 0.0 28 32.857142857142854 0.0 0.0 0.0 0.0 0.0 29 36.0 0.0 0.0 0.0 0.0 0.0 30 36.0 0.0 0.0 0.0 0.0 0.0 31 35.42857142857143 0.0 0.0 0.0 0.0 0.0 32 35.42857142857143 0.0 0.0 0.0 0.0 0.0 33 36.0 0.0 0.0 0.0 0.0 0.0 34 32.285714285714285 0.0 0.0 0.0 0.0 0.0 35 36.0 0.0 0.0 0.0 0.0 0.0 36 36.0 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 1.0 31 0.0 32 1.0 33 1.0 34 3.0 35 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.285714285714285 42.857142857142854 14.285714285714285 28.57142857142857 2 0.0 42.857142857142854 14.285714285714285 42.857142857142854 3 0.0 14.285714285714285 57.14285714285714 28.57142857142857 4 0.0 28.57142857142857 28.57142857142857 42.857142857142854 5 14.285714285714285 42.857142857142854 14.285714285714285 28.57142857142857 6 42.857142857142854 28.57142857142857 14.285714285714285 14.285714285714285 7 28.57142857142857 14.285714285714285 14.285714285714285 42.857142857142854 8 14.285714285714285 85.71428571428571 0.0 0.0 9 14.285714285714285 14.285714285714285 28.57142857142857 42.857142857142854 10 0.0 42.857142857142854 57.14285714285714 0.0 11 71.42857142857143 0.0 14.285714285714285 14.285714285714285 12 42.857142857142854 28.57142857142857 28.57142857142857 0.0 13 42.857142857142854 14.285714285714285 42.857142857142854 0.0 14 42.857142857142854 14.285714285714285 28.57142857142857 14.285714285714285 15 14.285714285714285 42.857142857142854 14.285714285714285 28.57142857142857 16 42.857142857142854 28.57142857142857 14.285714285714285 14.285714285714285 17 14.285714285714285 42.857142857142854 42.857142857142854 0.0 18 28.57142857142857 14.285714285714285 28.57142857142857 28.57142857142857 19 0.0 42.857142857142854 14.285714285714285 42.857142857142854 20 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 21 14.285714285714285 42.857142857142854 0.0 42.857142857142854 22 28.57142857142857 28.57142857142857 28.57142857142857 14.285714285714285 23 14.285714285714285 0.0 57.14285714285714 28.57142857142857 24 14.285714285714285 14.285714285714285 42.857142857142854 28.57142857142857 25 28.57142857142857 42.857142857142854 14.285714285714285 14.285714285714285 26 14.285714285714285 57.14285714285714 14.285714285714285 14.285714285714285 27 42.857142857142854 28.57142857142857 28.57142857142857 0.0 28 0.0 71.42857142857143 28.57142857142857 0.0 29 14.285714285714285 42.857142857142854 28.57142857142857 14.285714285714285 30 14.285714285714285 14.285714285714285 42.857142857142854 28.57142857142857 31 28.57142857142857 28.57142857142857 42.857142857142854 0.0 32 42.857142857142854 14.285714285714285 28.57142857142857 14.285714285714285 33 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 34 14.285714285714285 14.285714285714285 42.857142857142854 28.57142857142857 35 14.285714285714285 42.857142857142854 14.285714285714285 28.57142857142857 36 14.285714285714285 42.857142857142854 14.285714285714285 28.57142857142857 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.5 36 1.0 37 1.0 38 1.0 39 1.0 40 2.0 41 3.0 42 3.0 43 1.5 44 0.0 45 0.0 46 1.0 47 2.0 48 2.0 49 1.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 7.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTTAGTACTGATTGATCAGGGCAATGTAGTCCTTG 1 14.285714285714285 No Hit AATCAACAGAGGGTTTTGCTACTGAAAATTGGCCGT 1 14.285714285714285 No Hit GAACCAAACAGGTGAGAGCACTGTCGAATCTGAGAA 1 14.285714285714285 No Hit CCTTGTCACTCATGCAATTAAGTTTAGAGTGATCCC 1 14.285714285714285 No Hit TCTCAGCATTTTGGCGATCTCATCAAGAACTTTTAA 1 14.285714285714285 No Hit CCCACCGAAAGGGCACTAACAATTGCTACAATATAA 1 14.285714285714285 No Hit AACATGGGTTGTAAAGGCACCTCCGATTATAGGACC 1 14.285714285714285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE