FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728661
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT165180.6053518557270398No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC120760.44256138816804286No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC117610.431017264511788No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC96100.3521873915447906No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG93440.3424390204572865No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT88090.3228323342474569No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC87360.32015702940013435No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG83220.30498475259477087No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT74630.2735041106242219No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC73540.2695094773590417No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT63670.2333378899027765No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG60980.2234795747804509No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG59980.21981477361973512No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG58960.21607667643580497No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG56180.20588852920901496No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG56050.20541210505812194No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT54390.19932853513133364No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC52060.19078954842686577No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC50260.1841929063375773No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC50000.1832400580357912No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT49640.18192072961793349No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC48620.17818263243400334No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT48060.17613034378400247No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT45680.16740811702149883No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA44800.16418309200006892No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC43070.15784298599203053No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT42780.15678019365542292No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT42150.154471368924172No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC40110.14699517455631167No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA39880.14615227028934705No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA39020.14300054129113143No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC37070.13585417902773558No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT36850.1350479227723781No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG36730.1346081466330922No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG36560.1339851304357705No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT36220.13273909804112713No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT35220.1290742968804113No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG34500.1264356400446959No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT34230.12544614373130264No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT33740.12365039116255189No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA33670.12339385508130178No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC33460.12262424683755146No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT33250.12185463859380113No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA32740.11998559000183608No Hit
GTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTA32690.11980234994380028No Hit
CCGTTATCCGGATCGAGGACAGTGTATGGTGGGTAG31510.1154778845741556No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG31240.11448838826076232No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA30990.11357218797058337No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC30650.11232615557593999No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC30560.11199632347147558No Hit
GGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGA30410.11144660329736819No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC30380.11133665926254671No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC29880.1095042586821888No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA29710.10888124248486712No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC29680.10877129845004564No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT29520.10818493026433113No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG29190.10697554588129489No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC28830.10565621746343719No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC28810.10558292144022287No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA28590.10477666518486539No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG28520.10452012910361529No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC28410.10411700097593656No Hit
GCTAACTTTTCAAGGCCGAGAGCTGATGACGAGTGT28320.10378716887147212No Hit
GAGTTAGCCGGTGCTTATTCTGTCGGTAACGTCAAA27760.10173488022147126No Hit
ATCCGGATCGAGGACAGTGTATGGTGGGTAGTTTGA27440.1005621438500422No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA27290.10001242367593483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT31050.022.3184477
CCGTATG31300.022.1881058
CCACCGT31650.022.0403525
TATGCGC19200.021.8734111
CGTATGC32700.021.2381559
GTATGCG32700.021.23815510
CACCGTA32950.021.1248636
CTCGGTC5000.021.0023214
TATGCGT13400.020.59551811
ATCCACC33950.020.4576913
CATCCAC34000.020.427982
TCCACCG34300.020.4249974
ACTCGGT5150.020.3894823
ATGCGCA20600.020.31405612
TCATATA608.252804E-620.0014782
GTACCCG3700.019.8681551
CCGTGCT3950.019.369235
CAATAGT400.00650858118.7520734
GAGCCAT16650.018.741841
CCCGTGC4050.018.5205654
Produced by FastQC (version 0.11.2)