FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3748072
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT304770.8131380613819584No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC191200.510128940959512No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC152950.40807647238366823No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG132770.3542354575899289No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT107790.287587858504319No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG107370.28646728237878033No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC105470.2813980094299149No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT103550.2762753757131667No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG103250.27547496419492473No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC102710.27403422346208933No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG98930.2639490383322412No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC93630.24980843484330076No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG92200.24599313993968097No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT89520.2388427970433866No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG88360.23574787250618454No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT83830.22366165858073167No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC81190.2166180372202028No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG77810.2076000674480106No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC75590.20167702221302047No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA74170.19788840769334207No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC73480.1960474612013857No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT71080.18964416905545037No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC70360.1877231814116698No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT69040.18420137073140536No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT65400.17448971097673685No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG62510.16677908001767308No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC60550.16154972476515927No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC60260.16077599363085873No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT58220.15533319530681375No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT57400.1531454038236192No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT56490.15071748888495204No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT55910.149170026616351No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG54900.14647530783826992No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG53960.14396735174777858No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT52630.14041886068357276No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG51020.13612331886900783No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA50700.13526954658288315No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA50650.13513614466317617No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC50440.1345758566004068No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT50360.13436241352887565No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC49950.13326851778727836No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA49850.1330017139478644No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA49800.1328683120281574No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA49710.13262818857268482No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA49250.13140089091138057No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT48690.12990678941066233No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG48650.12980006787489676No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT47780.12747887447199518No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC47190.12590473181945278No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG46420.1238503422559652No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA45600.12166255077277065No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA45280.12080877848664594No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC44380.1184075439319202No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC44240.11803401855674064No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT44110.11768717356550248No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT43750.11672667974361219No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG41870.11171076756262954No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG41790.11149732449109837No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA41520.11077695412468062No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC41270.11010994452614571No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA40970.1093095330079038No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT40680.10853580187360327No Hit
CGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACC40390.10776207073930277No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC39650.10578772232763939No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT39480.10533415580063564No Hit
CTCCTCTACCGCATCATCAAAAGATGATACCCGTAG39200.10458710505027652No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC39190.10456042466633511No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC38930.10386673468385879No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC37770.10077181014665673No Hit
TCTTTTGATGATGCGGTAGAGGAGCGTTCTGTAAGC37510.1000781201641804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTC8750.024.000134
ACTCGGT9050.022.873053
TCTGACT5250.022.57004415
CTCTGAC5450.022.2922114
GAGCCAT18850.021.4068051
CCTCTGA5850.021.02435113
TATGCGC22500.020.86538511
GCCTCTG6200.020.80517412
CCACCGT41000.020.5244985
ACCCGTG5800.020.4311433
CCGTATG41200.020.3506938
ACCGTAT41100.020.2909977
GTACCCG6100.019.9189381
CGTATGC42600.019.752319
GTATGCG43050.019.72004310
CGTCTTT6250.019.6808931
CACCGTA43000.019.465226
CATACCC54700.019.1682933
CCATACC54900.019.1257862
AGAGGCA2750.019.09330430
Produced by FastQC (version 0.11.2)