FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662099
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT208220.7821647504469218No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC95480.35866434719369944No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC79390.29822331926799117No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG76920.28894492654104903No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG76170.2861276008142447No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG59150.22219308898729911No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT58130.21836152599884529No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC56620.21268931020221263No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT56150.2109237860800819No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG55730.20934608367307153No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG55040.20675414400441158No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC53990.2028098879868855No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT53260.20006769094612933No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA52940.1988656319693595No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG52820.19841485985307084No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC49620.1863942700853725No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT49600.18631914139932437No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT44950.16885172189313769No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT44390.1667481186837905No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC44000.16528310930585227No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT40060.15048275815437367No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG39480.14830402625897834No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC39230.1473649176833769No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC36780.13816165364248287No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG36760.13808652495643475No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC36190.1359453574040635No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC35870.13474329842729366No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA35600.13372906116564412No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT35240.13237674481677805No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA35090.13181327967141718No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT34890.13106199281093603No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG34810.13076147806674357No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT34710.130385834636503No Hit
GGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTT33740.12674209336316944No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG32610.12249732260145096No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA31740.11922922475835797No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT31610.11874088829904522No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT31580.11862819526997305No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT31470.11821498749670842No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC30840.11584843388619281No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA30680.1152474043978079No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC30490.11453368188035082No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC29390.1104016041477045No Hit
ATGTATCACTGTAGGGGGTAGAGCACTGTTTCGGCT29070.10919954517093466No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC28860.10841069396742946No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG28550.10724619933368369No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG28430.10679542721739499No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT28240.10608170469993791No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG27920.10487964572316807No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC27720.10412835886268693No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT27250.10236283474055624No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC26910.10108564707773829No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC26820.10074756799052177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTGA4900.021.12004313
CATACCC36250.020.773563
TATGCGC14000.020.35482411
GCCATAC37050.020.3288271
TCTGACT5000.020.09846515
CCATACC37100.020.0550142
TACCCTT36400.019.9461545
ACTCGGT5150.019.8069153
CGTCTTT5200.019.620151
CTCTGAC5300.019.5268114
CCGTATG20550.019.3408628
ACCGTAT20100.019.3265197
TAACTAG701.4462657E-619.2867772
ATACCCT39400.018.9604364
GCCTCTG5700.018.9452112
CCACCGT21300.018.9447065
CTTTCAT5500.018.8192184
GTGTTAA400.00651556918.74856814
TACCCTA651.6464526E-518.4625555
TTTAATG651.6464526E-518.4625552
Produced by FastQC (version 0.11.2)