FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296722
Sequences flagged as poor quality0
Sequence length36
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT13800.46508179373285435No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC10690.3602698822466821No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC10510.3542035979806014No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT8950.3016291343412352No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC6780.22849670735570668No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT6280.2116459177277047No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG5570.18771779645594192No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG5330.17962941743450098No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC5250.17693329109402067No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC5240.17659627530146063No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG5140.17322611737586022No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT5100.17187805420562008No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC4670.15738637512553838No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCT4650.1567123435404183No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT4630.15603831195529824No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC4450.14997202768921752No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA4330.14592783817849705No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT4250.14323171183801672No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT4170.14053558549753642No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG4130.13918752232729625No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC4050.13649139598681595No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG3810.128403016965375No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC3720.12536987483233464No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC3690.12435882745465453No Hit
ACCAGGAACCGTACCCCAAACCGACACAGGTGGTTG3420.11525940105553346No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA3410.11492238526297342No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT3370.11357432209273327No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT3330.11222625892249312No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC3320.11188924312993306No Hit
GGTACGCAGTCACCCAACAAAGTGGGCTCCCACTGC3270.11020416416713287No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG3180.10717102203409253No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT3120.10514892727873228No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG3110.10481191148617225No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC3070.10346384831593208No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC3050.102789816730812No Hit
CCCATTATACAAAAGGTACGCAGTCACCCAACAAAG3030.10211578514569193No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG2990.10076772197545178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT402.458159E-422.504551
ACCTAAA402.458159E-422.504551
TTAGATA350.002997438721.4329074
ACCGTAT2450.021.4292957
CCATGAT657.050494E-720.7734324
CCGTATG2550.020.585468
GTATGCG2600.020.18958710
AGTCACA608.227007E-619.99730312
TATGCGC1450.019.65252311
CACCGTA2700.019.4483786
CGTATGC2700.019.4418249
TATGCGT1251.8189894E-1219.1974111
CCACCGT2750.019.094775
GTCTCTT559.5464E-519.09155330
CTCAATA400.006491792418.7537922
TCCTATA400.006491792418.7537921
TAGATAC400.006491792418.7537925
CCTATAT400.006491792418.7537922
CATTCGT400.00649811518.75063121
TCTATTC1450.018.6244563
Produced by FastQC (version 0.11.2)