FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541689
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT30280.5589923369313388No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC14510.2678658787606911No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC14270.26343529220641365No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT13720.25328186468619446No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG12740.2351903029228949No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC12660.2337134407381357No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG11320.2089759991434199No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG11300.20860678359723014No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC10690.19734570943844162No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT10300.19014600628774075No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT8980.16577778023921474No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG7990.14750161070282025No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG7970.14713239515663046No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC7920.146209356291156No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC7610.14048651532521428No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG7530.13900965314045513No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG7410.1367943598633164No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC7150.13199455776284916No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC7100.13107151889737467No Hit
TCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGG6950.1283024023009513No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT6920.12774857898166658No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA6830.12608710902381257No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT6740.12442563906595852No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC6700.12368720797357893No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC6670.12313338465429426No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA6670.12313338465429426No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG6440.1188874058731117No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC6350.11722593591525765No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT6340.11704132814216274No Hit
GGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC6140.11334917268026488No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG5890.10873397835289252No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG5870.10836476280670274No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT5630.10393417625242528No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC5600.1033803529331406No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC5600.1033803529331406No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG5480.10116505965600188No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC5430.10024202079052741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACAGT200.00514070330.0049884
GGGTCTA1050.022.860946
TTAGGAC402.4624003E-422.5037430
CCGTATG3700.021.8914878
ACCGTAT3700.021.4841067
GTAGGAT350.003001230521.4321333
CCACCGT4050.020.0033255
CTTAGGA608.2327715E-620.00147629
TACCTTA608.2393435E-619.9996326
TATGCGC2950.019.83014311
CACCGTA4100.019.7593826
CGTATGC4100.019.7557339
CAGCTAT1750.019.71392325
CGGGTCT1251.8189894E-1219.2031925
ATGCGCA3150.019.04726812
GCGCACA3350.018.80562214
TAGGATA400.006499913518.7531194
GTATGCG4350.018.62034410
CTGAGAT1450.018.61862617
GATAGGG904.2471584E-818.3380741
Produced by FastQC (version 0.11.2)