FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478079
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT43540.9107281432566585No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC17490.36583911863938806No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC15980.33425438055216816No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG14370.30057793795586085No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG13390.2800792337668042No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT13350.27924255196316927No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG12160.2543512683050291No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT11130.23280671186142876No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT10420.21795560984690815No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC10340.2162822462396382No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC9570.20017612151966516No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC8900.18616170130877951No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC8680.18155995138878722No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG8370.17507566741061625No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC8260.1727747924506201No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT8110.169637235686989No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG7900.1652446562179054No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG7880.16482631531608793No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA7830.1637804630615442No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT7370.15415862231974214No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG7050.14746516789066244No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC7020.1468376565379362No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG6820.14265424751976138No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC6820.14265424751976138No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC6610.13826166805067783No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT5750.12027300927252609No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC5640.11797213431252994No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA5490.11483457754889882No Hit
GCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAA5460.11420706619617259No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC5420.11337038439253765No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG5370.11232453213799393No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT5370.11232453213799393No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT5350.11190619123617644No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG5270.11023282762890653No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT5260.11002365717799778No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA5170.1081411231198191No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC5170.1081411231198191No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC5110.10688610041436666No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC5100.10667692996345793No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA5070.1060494186107317No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC4800.1004018164361957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG300.001225506925.026
TATAGCC350.003003616121.4285723
TTCCTAG350.003003616121.4285723
GCCATAC7150.020.769231
ACTCGGT1101.8189894E-1220.4545463
CATACCC7550.020.26493
TACCCTT7500.020.0000025
CCATACC7600.019.934212
CTCGGTC1155.456968E-1219.5652164
ACCGTAT3950.018.987347
TCTATTC2450.018.9795913
ATACCCT8000.018.93754
CATTGTA400.00650506318.75000221
TCCTAGG400.00650506318.7500024
ATTCCTA400.00650506318.7500022
TGTAGCA400.00650506318.75000224
CACGTGT400.00650506318.75000229
CTGGGGA400.00650506318.7500022
GGTTAAA400.00650506318.75000221
ACCCTTG8100.018.7037036
Produced by FastQC (version 0.11.2)