FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289291
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATG16580.5731253305495159No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCA10490.36261065847191926No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGGCAT5680.19634209152721654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT1550.024.195361
ACAGCGC400.00650267118.7481638
AGGCATG2000.018.00134830
CTGGACC550.002095481416.3648624
GTATTAA755.5939832E-516.0011981
TCTTATA2850.015.2643012
TAGAACT600.00374301615.0011234
CTTGGAC851.6257478E-414.1187053
TATATCC750.001014139314.0010483
TGTATAC650.006374964513.8447988
GTTGTTC800.001652414613.1237148
TCTTGGA1056.711159E-512.8581072
ATAATAG954.1679776E-412.6325253
TAGGCAT2750.012.54639429
TTATACT850.002602652612.3538674
GTAATAA1101.03697384E-412.2736461
CTATAAT1006.419246E-412.0008981
ATACAGC900.003992466711.6675416
TACAGTG1059.666368E-411.4294285
TATACAA1059.666368E-411.4294285
Produced by FastQC (version 0.11.2)