##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HK5NHBGXX_combined_n01_ntr_8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8 Sequences flagged as poor quality 0 Sequence length 36 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0 0.0 0.0 0.0 0.0 0.0 2 32.0 0.0 0.0 0.0 0.0 0.0 3 32.0 0.0 0.0 0.0 0.0 0.0 4 29.75 0.0 0.0 0.0 0.0 0.0 5 29.75 0.0 0.0 0.0 0.0 0.0 6 36.0 0.0 0.0 0.0 0.0 0.0 7 29.5 0.0 0.0 0.0 0.0 0.0 8 32.25 0.0 0.0 0.0 0.0 0.0 9 34.375 0.0 0.0 0.0 0.0 0.0 10 33.25 0.0 0.0 0.0 0.0 0.0 11 36.0 0.0 0.0 0.0 0.0 0.0 12 36.0 0.0 0.0 0.0 0.0 0.0 13 36.0 0.0 0.0 0.0 0.0 0.0 14 35.5 0.0 0.0 0.0 0.0 0.0 15 33.25 0.0 0.0 0.0 0.0 0.0 16 33.25 0.0 0.0 0.0 0.0 0.0 17 35.5 0.0 0.0 0.0 0.0 0.0 18 36.0 0.0 0.0 0.0 0.0 0.0 19 36.0 0.0 0.0 0.0 0.0 0.0 20 35.5 0.0 0.0 0.0 0.0 0.0 21 34.5 0.0 0.0 0.0 0.0 0.0 22 33.25 0.0 0.0 0.0 0.0 0.0 23 36.0 0.0 0.0 0.0 0.0 0.0 24 32.25 0.0 0.0 0.0 0.0 0.0 25 30.5 0.0 0.0 0.0 0.0 0.0 26 35.5 0.0 0.0 0.0 0.0 0.0 27 33.625 0.0 0.0 0.0 0.0 0.0 28 35.5 0.0 0.0 0.0 0.0 0.0 29 36.0 0.0 0.0 0.0 0.0 0.0 30 34.875 0.0 0.0 0.0 0.0 0.0 31 35.5 0.0 0.0 0.0 0.0 0.0 32 32.75 0.0 0.0 0.0 0.0 0.0 33 36.0 0.0 0.0 0.0 0.0 0.0 34 36.0 0.0 0.0 0.0 0.0 0.0 35 35.0 0.0 0.0 0.0 0.0 0.0 36 35.5 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 33 4.0 34 3.0 35 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.0 12.5 25.0 37.5 2 12.5 25.0 50.0 12.5 3 12.5 25.0 37.5 25.0 4 0.0 37.5 37.5 25.0 5 12.5 37.5 50.0 0.0 6 37.5 25.0 12.5 25.0 7 25.0 50.0 12.5 12.5 8 12.5 37.5 0.0 50.0 9 25.0 12.5 0.0 62.5 10 0.0 50.0 37.5 12.5 11 62.5 0.0 25.0 12.5 12 50.0 12.5 12.5 25.0 13 37.5 12.5 37.5 12.5 14 12.5 25.0 12.5 50.0 15 37.5 12.5 25.0 25.0 16 50.0 25.0 25.0 0.0 17 25.0 12.5 50.0 12.5 18 12.5 12.5 37.5 37.5 19 0.0 12.5 50.0 37.5 20 37.5 37.5 25.0 0.0 21 37.5 25.0 25.0 12.5 22 0.0 37.5 37.5 25.0 23 25.0 37.5 25.0 12.5 24 50.0 25.0 25.0 0.0 25 0.0 37.5 50.0 12.5 26 37.5 12.5 12.5 37.5 27 0.0 37.5 50.0 12.5 28 25.0 25.0 12.5 37.5 29 12.5 37.5 25.0 25.0 30 25.0 25.0 37.5 12.5 31 0.0 37.5 37.5 25.0 32 12.5 37.5 37.5 12.5 33 37.5 12.5 12.5 37.5 34 62.5 0.0 12.5 25.0 35 0.0 50.0 12.5 37.5 36 37.5 25.0 37.5 0.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.5 39 1.0 40 2.0 41 3.0 42 3.0 43 2.0 44 1.0 45 1.0 46 1.0 47 1.0 48 1.0 49 1.0 50 1.0 51 0.5 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.5 61 1.0 62 1.0 63 0.5 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 8.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTACTTCGGTGGTAGGGAAAGATATCTGCACCGGAT 1 12.5 No Hit AGCCAAACAAGAGCAGTTCTCAGATTACAATGGGAG 1 12.5 No Hit TTTATGAACCTCTCCACTTTAAAGTACGACAACGAG 1 12.5 No Hit GTATTCGCCATCGCGAGCCAGCTGACTCCGAAGGCA 1 12.5 No Hit CATAGGTACTGGCATTAGTGTTGGAGTTGGTACTTT 1 12.5 No Hit TAGTTAGAGAGGATGGTCTGGCCTTCTATTATACCT 1 12.5 No Hit GCTAACACCTGTTCCTTCCGATATAGACATCTCCAG 1 12.5 No Hit CTCTAGACCACGGGTGTTTATTAGCGAATTTTTGCA 1 12.5 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE