FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823165
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGG4750.019.8937510
GAGCCCG5000.019.1990479
CGGCCCG5650.019.11409814
CCCGGCC5950.018.40212412
GCCCGGG5950.017.8995425
CCCGGGG5400.017.7784726
GGAGCCC5450.017.6138068
GGGCCCG6050.017.35512523
CCGGCCC6500.017.07577513
GCCCGGC6600.016.81704911
CGGAGGG6300.016.6658419
CCGGGGC5600.016.60749427
CCCGGAG6350.016.29840517
AGGAGCC6250.015.1192517
GCCCGGA6850.015.1087416
GAGGGCC7450.014.295121
AGGGCCC7600.014.01295922
CCGGAGG7850.013.37513318
AAGGAGC8450.013.3148356
GGAGGGC8150.012.88325320
Produced by FastQC (version 0.11.2)