FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4076536
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC3950.010.2524788
ACTGTGC5550.09.999338
TATACCG6300.09.7619695
TATACGG11000.09.6818825
CTAATAC10250.09.5122583
CCTGAGC5200.09.23196230
TAGGGGG2957.943527E-99.1526024
CCGAGCC5250.09.14235714
CTCTTAT12550.09.08439351
CATATAC20100.08.8806543
CAGCGCA3758.185452E-118.7994099
CTCCGAG5350.08.691112512
GGTCGGG1901.820006E-48.68373414
GCCCACG5750.08.60843518
TATTATA25100.08.5458732
TCTTATA16400.08.5061532
TCTAATA15000.08.5000552
ACAGTGC4950.08.484288
TATACTG11500.08.4783165
TGGGGGG5150.08.4466575
Produced by FastQC (version 0.11.2)