FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4860700
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG4850.012.0628115
CTGCGCG1355.6590678E-511.1105059
AGTCCGG6100.011.06485710
CTGAGCG2453.739842E-910.4075979
TAGGGGG3500.010.286534
GTATTAG9800.010.2565481
CTAATAC12250.010.1640693
GAGCCGG7700.09.54611329
CGATCAC3203.4197E-109.37487424
GCCCGAC1450.00134066769.30974111
TATAATA32500.09.0929342
TAATACT20200.09.0601234
ATTATAC16950.09.0272643
AGGGGGG6150.09.0251055
TAGACTG5200.08.9430165
CCTAATA13300.08.9103892
TTATACT17550.08.8895934
CCTATAC7450.08.8597623
GCCCGCC6100.08.85188516
GTATAAA19500.08.8474011
Produced by FastQC (version 0.11.2)