FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4370837
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGG1302.7654078E-612.6914915
CGGTCGG1354.218962E-612.22129513
TGGGGGG5250.011.7147375
TAGGGGG2950.011.1868724
GGTAGTC4750.010.7362737
ACGGCCG1000.00877745810.50100529
GTCTTAG6000.010.0016441
GCCGCGT3001.0004442E-1010.00107225
GTATTAG10550.09.9542441
TAGGTAG5750.09.6525455
TATTAGG10800.09.5837032
AGGGGGG4400.09.5458225
CTATGCA9400.09.4141799
CTGTCCG1654.2893612E-49.09021959
TATACGG9250.09.0814315
CTAATAC11900.09.0758763
ACTATGC11800.09.0247388
CTGCCCC3001.0610165E-88.9993189
CTCTTAT8550.08.9488391
CTCCGAG4700.08.93559414
Produced by FastQC (version 0.11.2)