FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805659
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCC1903.6379788E-1214.20983114
TGGGGGG2250.013.3348965
CGAGCCC2153.274181E-1112.55752515
AGTCCGA1101.0437418E-412.27246710
GCCTAGA1252.5731872E-512.0010741
AGTCCGG2301.05501385E-1011.73888110
GCCCACG2301.05501385E-1011.73855718
CGAGACT2503.8198777E-1111.39944223
GCCCGCC1859.170435E-811.35079616
ACAGTCC1202.3459822E-411.2497618
GAGTCCG1202.3459822E-411.2497619
CGGCCCG2002.2293534E-811.2494514
GGCCCGC1901.3262252E-711.05209115
TCCTACA2453.1832315E-1011.0213962
GTATTAG3750.010.8009671
CCCACGA2504.5474735E-1010.79947119
ACGAGAC2802.7284841E-1110.71376122
TCCGAGC2402.6557245E-910.62447913
CCGGCCC1851.1218144E-610.54002513
CCCGCCC1851.1218144E-610.54002517
Produced by FastQC (version 0.11.2)